:root {
  --bg: #0c0f14;
  --bg-elevated: #141a24;
  --bg-input: #0e131c;
  --border: rgba(94, 234, 212, 0.12);
  --accent: #5eead4;
  --accent-dim: rgba(94, 234, 212, 0.55);
  --text: #e8edf5;
  --text-muted: #8b98ab;
  --danger: #f87171;
  --font-sans: "DM Sans", system-ui, sans-serif;
  --font-mono: "JetBrains Mono", "Courier New", monospace;
  --radius: 12px;
  --shadow: 0 24px 48px rgba(0, 0, 0, 0.45);
}

*,
*::before,
*::after {
  box-sizing: border-box;
}

body {
  margin: 0;
  min-height: 100vh;
  font-family: var(--font-sans);
  font-size: 16px;
  line-height: 1.5;
  color: var(--text);
  background:
    radial-gradient(ellipse 120% 80% at 50% -20%, rgba(94, 234, 212, 0.08), transparent),
    radial-gradient(ellipse 80% 50% at 100% 50%, rgba(56, 189, 248, 0.06), transparent),
    var(--bg);
}

main {
  max-width: 960px;
  margin: 0 auto;
  padding: 2.5rem 1.25rem 4rem;
}

header.hero {
  text-align: center;
  margin-bottom: 2rem;
}

header.hero h1 {
  margin: 0 0 0.35rem;
  font-weight: 700;
  font-size: clamp(1.65rem, 4vw, 2.15rem);
  letter-spacing: -0.03em;
  background: linear-gradient(135deg, #f0fdfa 0%, var(--accent) 55%, #38bdf8 100%);
  -webkit-background-clip: text;
  background-clip: text;
  color: transparent;
}

.pg-abbr {
  font-weight: 600;
  opacity: 0.85;
}

header.hero .hero-guide {
  max-width: 44rem;
  margin: 0.75rem auto 0;
  text-align: left;
}

header.hero .hero-guide p {
  margin: 0;
  color: var(--text-muted);
  font-size: 0.95rem;
  line-height: 1.58;
}

header.hero .hero-guide p + p {
  margin-top: 0.85rem;
}

header.hero .hero-guide a {
  color: var(--accent);
  font-weight: 600;
}

.panel {
  background: var(--bg-elevated);
  border: 1px solid var(--border);
  border-radius: var(--radius);
  padding: 1.35rem;
  box-shadow: var(--shadow);
}

.panel + .panel {
  margin-top: 1.25rem;
}

.field-label {
  display: block;
  font-size: 0.72rem;
  font-weight: 600;
  letter-spacing: 0.12em;
  text-transform: uppercase;
  color: var(--accent-dim);
  margin-bottom: 0.45rem;
}

.field-hint {
  margin: 0.35rem 0 0;
  font-size: 0.72rem;
  color: var(--text-muted);
  line-height: 1.4;
}

.form-grid {
  display: grid;
  grid-template-columns: 1fr 1fr;
  gap: 1rem;
}

.form-span-2 {
  grid-column: 1 / -1;
}

@media (max-width: 640px) {
  .form-grid {
    grid-template-columns: 1fr;
  }
}

.pg-select,
.pg-input {
  width: 100%;
  padding: 0.65rem 0.85rem;
  font-family: var(--font-sans);
  font-size: 0.9rem;
  color: var(--text);
  background: var(--bg-input);
  border: 1px solid var(--border);
  border-radius: 10px;
}

.pg-input[type="number"] {
  font-family: var(--font-mono);
}

.pg-select:focus,
.pg-input:focus {
  outline: none;
  border-color: rgba(94, 234, 212, 0.45);
  box-shadow: 0 0 0 3px rgba(94, 234, 212, 0.12);
}

.toolbar {
  margin-top: 1rem;
}

.toolbar--secondary {
  margin-top: 0.65rem;
}

.btn-fetch {
  padding: 0.65rem 1.35rem;
  font-family: var(--font-sans);
  font-weight: 600;
  font-size: 0.9rem;
  color: #042f2e;
  background: linear-gradient(135deg, #5eead4 0%, #2dd4bf 50%, #22d3ee 100%);
  border: none;
  border-radius: 10px;
  cursor: pointer;
  box-shadow: 0 4px 14px rgba(45, 212, 191, 0.35);
}

.btn-fetch:hover:not(:disabled) {
  transform: translateY(-1px);
}

.btn-fetch:disabled {
  opacity: 0.65;
  cursor: wait;
}

.btn-secondary {
  padding: 0.5rem 1rem;
  font-family: var(--font-sans);
  font-weight: 600;
  font-size: 0.85rem;
  color: var(--text);
  background: var(--bg-input);
  border: 1px solid var(--border);
  border-radius: 8px;
  cursor: pointer;
}

.btn-secondary:hover {
  border-color: rgba(94, 234, 212, 0.45);
}

.stats-row {
  margin-top: 0.85rem;
  font-size: 0.82rem;
  color: var(--text-muted);
}

.stats-row .error {
  color: var(--danger);
}

.pg-pick-list {
  display: flex;
  flex-direction: column;
  gap: 0.5rem;
}

.pg-pick-btn {
  text-align: left;
  padding: 0.75rem 1rem;
  font-family: var(--font-sans);
  font-size: 0.88rem;
  color: var(--text);
  background: var(--bg-input);
  border: 1px solid var(--border);
  border-radius: 10px;
  cursor: pointer;
}

.pg-pick-btn:hover {
  border-color: rgba(94, 234, 212, 0.45);
  background: rgba(94, 234, 212, 0.06);
}

.pg-pick-btn strong {
  color: var(--accent);
}

.pg-pick-btn small {
  color: var(--text-muted);
}

.pg-meta-grid {
  display: grid;
  grid-template-columns: repeat(auto-fill, minmax(220px, 1fr));
  gap: 0.65rem 1.25rem;
  margin: 0;
  font-size: 0.85rem;
}

.pg-meta-grid dt {
  margin: 0;
  font-size: 0.68rem;
  font-weight: 600;
  letter-spacing: 0.1em;
  text-transform: uppercase;
  color: var(--accent-dim);
}

.pg-meta-grid dd {
  margin: 0.15rem 0 0;
  color: var(--text);
}

.pg-map-toolbar {
  display: flex;
  flex-wrap: wrap;
  align-items: center;
  gap: 0.5rem 0.75rem;
  margin: 0.35rem 0 0.5rem;
}

.pg-view-range {
  margin: 0;
  flex: 1 1 12rem;
  font-size: 0.8rem;
  color: var(--muted);
}

.pg-diagram-viewport {
  margin: 0 0 1.25rem;
  padding: 1rem;
  background: #f8fafc;
  border: 1px solid var(--border);
  border-radius: 10px;
  overflow-x: auto;
  cursor: grab;
  touch-action: none;
  user-select: none;
}

.pg-diagram-viewport.is-dragging {
  cursor: grabbing;
}

.pg-diagram-viewport.is-loading {
  opacity: 0.72;
  pointer-events: none;
}

.pg-diagram-inner {
  min-width: 640px;
}

.pg-diagram-svg {
  display: block;
  min-width: 640px;
  width: 100%;
  height: auto;
}

.pg-diagram-svg .pg-label {
  font-family: var(--font-sans);
  font-size: 11px;
  fill: #334155;
}

.pg-diagram-svg .pg-muted {
  font-family: var(--font-sans);
  font-size: 10px;
  fill: #64748b;
}

.pg-diagram-svg .pg-chr-title {
  font-family: var(--font-sans);
  font-size: 12px;
  font-weight: 700;
  fill: #0f172a;
}

.pg-diagram-svg .pg-dna-line {
  stroke: #475569;
  stroke-width: 2;
}

.pg-diagram-svg .pg-axis {
  stroke: #cbd5e1;
  stroke-width: 1;
}

.pg-diagram-svg .pg-exon {
  fill: #64748b;
  stroke: #475569;
  stroke-width: 1;
}

.pg-diagram-svg .pg-exon--target {
  fill: #2563eb;
  stroke: #1e40af;
  stroke-width: 1.5;
}

.pg-diagram-svg .pg-exon--neighbor {
  fill: #94a3b8;
  stroke: #64748b;
}

.pg-diagram-svg .pg-intron {
  stroke: #94a3b8;
  stroke-width: 2;
}

.pg-diagram-svg .pg-intron--target {
  stroke: #64748b;
}

.pg-diagram-svg .pg-intron--thin {
  stroke: #94a3b8;
  stroke-width: 1.5;
  stroke-linecap: round;
}

.pg-diagram-svg .pg-promoter-band {
  fill: rgba(94, 234, 212, 0.55);
  stroke: none;
}

.pg-diagram-svg .pg-intron-label {
  font-family: var(--font-sans);
  font-size: 8px;
  fill: #94a3b8;
  font-style: italic;
}

.pg-diagram-svg .pg-exon-label {
  font-family: var(--font-sans);
  font-size: 8px;
  fill: #fff;
  font-weight: 600;
}

.pg-diagram-svg .pg-gene-label {
  font-family: var(--font-sans);
  font-size: 11px;
  font-weight: 600;
  fill: #334155;
}

.pg-diagram-svg .pg-gene-label--target {
  fill: #1d4ed8;
  font-weight: 700;
}

.pg-diagram-svg .pg-gene-strand {
  font-family: var(--font-sans);
  font-size: 9px;
  fill: #64748b;
}

.pg-diagram-svg .pg-strand-chevron {
  fill: rgba(15, 118, 110, 0.35);
}

.pg-diagram-svg .pg-target-highlight {
  fill: rgba(94, 234, 212, 0.2);
  stroke: rgba(13, 148, 136, 0.5);
  stroke-width: 1;
}

.pg-diagram-svg .pg-legend {
  font-family: var(--font-sans);
  font-size: 9px;
  fill: #475569;
}

.pg-diagram-svg .pg-intron-implied {
  fill: none;
}

.pg-diagram-svg .pg-tss {
  stroke: #dc2626;
  stroke-width: 2;
  stroke-dasharray: 4 3;
}

.pg-diagram-svg .pg-tss-label {
  font-family: var(--font-sans);
  font-size: 10px;
  font-weight: 700;
  fill: #dc2626;
}

.pg-diagram-svg .pg-scale {
  font-family: var(--font-mono);
  font-size: 9px;
  fill: #64748b;
}

.pg-diagram-svg .pg-region-band {
  fill: rgba(94, 234, 212, 0.35);
}

.pg-diagram-svg .pg-arrow-line {
  stroke: #0d9488;
  stroke-width: 3;
}

.pg-diagram-svg .pg-arrow-head {
  fill: #0d9488;
}

.pg-diagram-svg .pg-arrow-label {
  font-family: var(--font-sans);
  font-size: 10px;
  font-weight: 600;
  fill: #0f766e;
}

.pg-primer-section {
  display: block;
  margin: 1.25rem 0 1.5rem;
  border: 1px solid var(--border);
  border-radius: var(--radius);
  background: var(--bg-elevated);
  box-shadow: var(--shadow);
  overflow: hidden;
}

#resultsPanel:not([hidden]) .pg-primer-section {
  display: block;
}

.pg-reporter-content {
  padding-top: 1rem;
  font-size: 0.9rem;
  line-height: 1.58;
  color: var(--text-muted);
}

.pg-reporter-content p {
  margin: 0 0 0.85rem;
}

.pg-reporter-content p:last-child {
  margin-bottom: 0;
}

.pg-reporter-content strong {
  color: var(--text);
}

.pg-reporter-content a {
  color: var(--accent);
  font-weight: 600;
  text-decoration: none;
}

.pg-reporter-content a:hover {
  text-decoration: underline;
}

.pg-reporter-figures {
  display: grid;
  grid-template-columns: repeat(2, minmax(0, 1fr));
  gap: 1rem;
  margin: 1rem 0;
}

.pg-reporter-figure {
  margin: 0;
  padding: 0.65rem;
  border: 1px solid var(--border);
  border-radius: var(--radius);
  background: rgba(8, 12, 18, 0.55);
}

.pg-reporter-zoom {
  display: block;
  width: 100%;
  margin: 0;
  padding: 0;
  border: none;
  background: transparent;
  cursor: zoom-in;
  border-radius: 6px;
}

.pg-reporter-zoom:focus-visible {
  outline: 2px solid var(--accent);
  outline-offset: 3px;
}

.pg-reporter-zoom img {
  display: block;
  width: 100%;
  max-height: min(280px, 42vw);
  object-fit: contain;
  border-radius: 6px;
  pointer-events: none;
}

.pg-reporter-zoom-hint {
  margin: -0.35rem 0 0.75rem;
  font-size: 0.75rem;
  color: var(--text-muted);
  text-align: center;
}

.pg-reporter-figure figcaption {
  margin-top: 0.5rem;
  font-size: 0.78rem;
  color: var(--text-muted);
  text-align: center;
}

.pg-reporter-links {
  font-size: 0.86rem;
}

@media (max-width: 720px) {
  .pg-reporter-figures {
    grid-template-columns: 1fr;
  }

  .pg-reporter-zoom img {
    max-height: 200px;
  }
}

.pg-lightbox {
  position: fixed;
  inset: 0;
  z-index: 10000;
  display: flex;
  align-items: center;
  justify-content: center;
  padding: clamp(0.75rem, 3vw, 1.5rem);
}

.pg-lightbox[hidden] {
  display: none !important;
}

.pg-lightbox-backdrop {
  position: absolute;
  inset: 0;
  margin: 0;
  padding: 0;
  border: none;
  background: rgba(4, 8, 14, 0.92);
  cursor: zoom-out;
}

.pg-lightbox-panel {
  position: relative;
  z-index: 1;
  display: flex;
  flex-direction: column;
  align-items: center;
  gap: 0.65rem;
  max-width: min(96vw, 1100px);
  max-height: 94vh;
}

.pg-lightbox-panel img {
  display: block;
  max-width: 100%;
  max-height: calc(94vh - 3.5rem);
  width: auto;
  height: auto;
  object-fit: contain;
  border-radius: 8px;
  background: #fff;
  box-shadow: 0 12px 40px rgba(0, 0, 0, 0.55);
}

.pg-lightbox-close {
  position: absolute;
  top: -0.35rem;
  right: -0.35rem;
  z-index: 2;
  width: 2.25rem;
  height: 2.25rem;
  border: 1px solid var(--border);
  border-radius: 999px;
  background: rgba(12, 18, 28, 0.95);
  color: var(--text);
  font-size: 1.35rem;
  line-height: 1;
  cursor: pointer;
}

.pg-lightbox-close:hover {
  border-color: var(--accent);
  color: var(--accent);
}

.pg-lightbox-caption {
  margin: 0;
  max-width: 100%;
  font-size: 0.82rem;
  color: var(--text-muted);
  text-align: center;
}

body.pg-lightbox-open {
  overflow: hidden;
}

.pg-primer-toggle {
  display: flex;
  align-items: center;
  gap: 0.65rem;
  width: 100%;
  margin: 0;
  padding: 0.95rem 1.1rem;
  border: none;
  background: transparent;
  color: var(--accent);
  font-family: var(--font-sans);
  font-size: 0.82rem;
  font-weight: 700;
  letter-spacing: 0.06em;
  text-transform: uppercase;
  text-align: left;
  cursor: pointer;
  transition: background 0.15s ease;
}

.pg-primer-toggle:hover {
  background: rgba(94, 234, 212, 0.06);
}

.pg-primer-toggle:focus-visible {
  outline: none;
  box-shadow: inset 0 0 0 2px rgba(94, 234, 212, 0.45);
}

.pg-primer-chevron {
  flex: 0 0 auto;
  display: inline-block;
  font-size: 1.15rem;
  line-height: 1;
  color: var(--accent);
  transition: transform 0.2s ease;
}

.pg-primer-toggle[aria-expanded="true"] .pg-primer-chevron {
  transform: rotate(90deg);
}

.pg-primer-body {
  padding: 0 1.1rem 1.1rem;
  border-top: 1px solid var(--border);
}

.pg-primer-out {
  padding-top: 1rem;
}

.pg-primer-set + .pg-primer-set {
  margin-top: 1.25rem;
}

.pg-primer-set-title {
  margin: 0 0 0.6rem;
  font-size: 0.95rem;
  font-weight: 700;
  color: var(--text);
  letter-spacing: -0.01em;
}

.pg-primer-table {
  width: 100%;
  border-collapse: collapse;
  font-size: 0.85rem;
}

.pg-primer-table th,
.pg-primer-table td {
  padding: 0.5rem 0.65rem;
  text-align: left;
  vertical-align: top;
  border-bottom: 1px solid var(--border);
}

.pg-primer-table th {
  font-weight: 600;
  color: var(--text-muted);
  white-space: nowrap;
}

.pg-primer-seq code {
  font-family: var(--font-mono);
  font-size: 0.8rem;
  color: var(--accent);
  word-break: break-all;
}

.pg-primer-error {
  margin: 0 0 0.75rem;
  color: var(--danger);
  font-size: 0.88rem;
}

.pg-primer-note {
  margin: 1rem 0 0;
  font-size: 0.78rem;
  color: var(--text-muted);
  line-height: 1.5;
}

.pg-cloning-sites {
  margin-top: 1.5rem;
  padding-top: 1.25rem;
  border-top: 1px solid var(--border);
}

.pg-cloning-lead {
  margin: 0 0 0.65rem;
  font-size: 0.88rem;
  color: var(--text-muted);
  line-height: 1.5;
}

.pg-cloning-legend {
  margin: 0 0 1rem;
  font-size: 0.82rem;
  color: var(--text-muted);
}

.pg-cloning-picks {
  display: grid;
  grid-template-columns: 1fr 1fr;
  gap: 1rem;
  margin-bottom: 1rem;
}

.pg-cloning-pick {
  display: flex;
  flex-direction: column;
  gap: 0.35rem;
}

.pg-cloning-label {
  font-size: 0.82rem;
  font-weight: 600;
  color: var(--text-muted);
}

.pg-clone-select {
  width: 100%;
  font-size: 0.88rem;
}

.pg-clone-select--zero {
  color: #4ade80;
  border-color: rgba(74, 222, 128, 0.45);
}

.pg-clone-select--cut {
  color: #f87171;
  border-color: rgba(248, 113, 113, 0.45);
}

.pg-clone-select option.pg-re-opt--zero {
  color: #4ade80;
  background: #0e131c;
}

.pg-clone-select option.pg-re-opt--cut {
  color: #f87171;
  background: #0e131c;
}

.pg-cloning-subhead {
  margin: 1rem 0 0.5rem;
  font-size: 0.88rem;
  font-weight: 700;
  color: var(--text);
}

.pg-clone-detail {
  margin-bottom: 0.75rem;
}

.pg-clone-hint {
  margin: 0;
  font-size: 0.85rem;
  color: var(--text-muted);
}

.pg-clone-detail-list {
  margin: 0;
  padding: 0;
  list-style: none;
}

.pg-clone-detail-list li {
  margin: 0 0 0.65rem;
  padding: 0.65rem 0.75rem;
  border-radius: 8px;
  font-size: 0.85rem;
  line-height: 1.5;
  border: 1px solid var(--border);
}

.pg-clone-detail-item--zero {
  background: rgba(74, 222, 128, 0.08);
  border-color: rgba(74, 222, 128, 0.28);
}

.pg-clone-detail-item--cut {
  background: rgba(248, 113, 113, 0.08);
  border-color: rgba(248, 113, 113, 0.28);
}

.pg-clone-pos {
  font-size: 0.8rem;
  color: var(--text-muted);
}

.pg-re-site {
  font-family: var(--font-mono);
  font-size: 0.78rem;
  color: var(--accent);
}

.pg-re-badge {
  display: inline-block;
  padding: 0.1rem 0.45rem;
  border-radius: 4px;
  font-size: 0.72rem;
  font-weight: 700;
  letter-spacing: 0.02em;
  vertical-align: middle;
}

.pg-re-badge--zero {
  color: #4ade80;
  background: rgba(74, 222, 128, 0.15);
}

.pg-re-badge--cut {
  color: #f87171;
  background: rgba(248, 113, 113, 0.15);
}

.pg-re-table-wrap {
  overflow-x: auto;
}

.pg-re-scan-table .pg-re-row--zero td {
  color: #bbf7d0;
}

.pg-re-scan-table .pg-re-row--cut td {
  color: #fecaca;
}

.pg-re-scan-table .pg-re-row--zero .pg-re-badge--zero,
.pg-re-scan-table .pg-re-row--cut .pg-re-badge--cut {
  color: inherit;
}

.pg-pcr-suggest {
  margin-top: 1.5rem;
  padding-top: 1.25rem;
  border-top: 1px solid var(--border);
}

.pg-pcr-block + .pg-pcr-block {
  margin-top: 1rem;
}

.pg-pcr-subhead {
  margin: 0 0 0.45rem;
  font-size: 0.9rem;
  font-weight: 700;
  color: var(--text);
}

.pg-pcr-meta {
  margin: 0 0 0.35rem;
  font-size: 0.82rem;
  color: var(--text-muted);
  line-height: 1.45;
}

.pg-pcr-plan-list {
  margin: 0.6rem 0 0;
  padding-left: 1.1rem;
  font-size: 0.84rem;
  color: var(--text);
  line-height: 1.45;
}

.pg-pcr-plan-list li + li {
  margin-top: 0.2rem;
}

.pg-pcr-stepdown-toggle {
  display: inline-flex;
  align-items: center;
  gap: 0.5rem;
  margin-top: 0.65rem;
  font-size: 0.84rem;
  color: var(--text-muted);
}

.pg-pcr-stepdown-toggle input[type="checkbox"] {
  width: 1rem;
  height: 1rem;
  accent-color: var(--accent);
}

@media (max-width: 640px) {
  .pg-cloning-picks {
    grid-template-columns: 1fr;
  }

  .pg-primer-table {
    font-size: 0.8rem;
  }
  .pg-primer-table th:nth-child(3),
  .pg-primer-table td:nth-child(3) {
    display: none;
  }
}

.seq-output {
  width: 100%;
  min-height: 140px;
  padding: 0.85rem 1rem;
  font-family: var(--font-mono);
  font-size: 0.78rem;
  line-height: 1.55;
  color: var(--text);
  background: var(--bg-input);
  border: 1px solid var(--border);
  border-radius: 10px;
  resize: vertical;
}

.seq-output--pretty {
  margin: 0;
  white-space: pre-wrap;
  word-break: break-word;
  overflow-wrap: anywhere;
  resize: none;
}

.pg-seq-tss-plus1 {
  color: #fca5a5;
  font-weight: 700;
}

.pg-seq-downstream {
  color: #fde68a;
}

.pg-seq-inner-amplicon {
  background: rgba(56, 189, 248, 0.22);
  border-radius: 3px;
  box-decoration-break: clone;
  -webkit-box-decoration-break: clone;
  padding: 0.05em 0;
}

.pg-seq-inner-amplicon .pg-seq-downstream {
  color: #fef9c3;
}

.pg-seq-inner-amplicon .pg-seq-tss-plus1 {
  color: #fecaca;
}

.pg-seq-legend {
  margin: 0.5rem 0 0;
  display: flex;
  flex-wrap: wrap;
  gap: 0.4rem 0.9rem;
  font-size: 0.76rem;
  color: var(--text-muted);
}

.pg-seq-legend-item {
  display: inline-flex;
  align-items: center;
  gap: 0.4rem;
}

.pg-seq-legend-chip {
  width: 0.9rem;
  height: 0.7rem;
  border-radius: 3px;
  border: 1px solid var(--border);
  display: inline-block;
}

.pg-seq-legend-chip--tss {
  background: rgba(252, 165, 165, 0.3);
  border-color: rgba(252, 165, 165, 0.55);
}

.pg-seq-legend-chip--downstream {
  background: rgba(253, 230, 138, 0.32);
  border-color: rgba(253, 230, 138, 0.55);
}

.pg-seq-legend-chip--inner {
  background: rgba(56, 189, 248, 0.22);
  border-color: rgba(56, 189, 248, 0.5);
}

footer.note {
  margin-top: 2rem;
  font-size: 0.75rem;
  color: var(--text-muted);
  text-align: center;
  line-height: 1.6;
}

@media (max-width: 640px) {
  main {
    padding: 1.4rem 0.85rem 2rem;
  }
}