DNA translation

This tool translates a DNA sequence into amino acid sequences in all three forward reading frames. It helps you find open reading frames, check whether a construct is in frame, or compare how translation shifts when the sequence starts at different positions.

Paste DNA (ACGT; FASTA headers are skipped) or upload a FASTA file. Choose single-letter or three-letter amino acid codes. Stop codons are shown in the output so you can spot where translation terminates in each frame.

Translation runs entirely in your browser. This is a simple codon table lookup — it does not predict splice sites, RNA editing, or selenocysteine exceptions. For reverse-strand genes, reverse-complement your sequence first using the DNA reverse & complement tool, then translate here.