DNA reverse & reverse complement

This tool converts a DNA sequence into the reverse sequence (3′→5′ order of the same strand) or the reverse complement (the opposite strand sequence, 5′→3′). These operations are routine when designing primers, annotating features on the antisense strand, or preparing sequence for cloning and assembly.

Paste DNA or load a FASTA file, select the operation, and read the result in the output box. Use A, C, G, and T for standard bases. FASTA header lines are ignored; sequence lines are concatenated.

For cloning, the reverse complement is often what you need when a feature is drawn on the antisense strand but you want the sequence to order or anneal primers. Processing is local in your browser with no account required.