Restriction site analyzer

Restriction digest analysis is essential when trying to clone a fragment of DNA into a plasmid. Typically, you would include restriction sites in the forward and reverse PCR primers that you used to amplify the DNA fragment you aim to clone. Of course, it is essential that you do not already have one or more of the chosen recognition sites within the main sequence of your DNA fragment, otherwise the restriction enzyme will cut your DNA into two or more fragments and not just create sticky ends for cloning.

Paste your sequence in the box below and click Analyze. Choose zero cutters from the drop-down menu to see a list of restriction enzymes that do not cut your sequence.